why we should use only 0.45 micron filter in sterility test, 0.1 micron size bacteria also present
Because Sterility test is a recovery test as during manufacturing there is a filtration process which uses 0.2 micron filter and moreover many microorganisms get killed during autoclaving process.
Dear vivek Sorry i am not understand what u say,Please clarify in detail
During mfg process there is a step known as filtration in which 0.2 micron filters are used which is known as retention step. In sterility we have to find out the microorganisms not to remove them. This is known as recovery test.
As Vivek mentioned above it is a recovery test where 0.4 micron can retain the microbes whreas 0.2 micron can not retain microbs in the pores.