I jz knew I practice the wrong method for some time. From the usp it is stated ‘…use the quantity corresponding to 10 g/mL to inoculate suitable amount of TSB…’.I did transfer 10 mL from mixture of sample+peptone into TSB. But am I wrong to dissolve 10 g sample into 90 mL peptone? How many sample should I use?
Really need an answer.
Thanks in advance.