Hi
I jz knew I practice the wrong method for some time. From the usp it is stated ‘…use the quantity corresponding to 10 g/mL to inoculate suitable amount of TSB…’.I did transfer 10 mL from mixture of sample+peptone into TSB. But am I wrong to dissolve 10 g sample into 90 mL peptone? How many sample should I use?
Really need an answer.
Thanks in advance.
To get per 10g of sample, take 10g sample + 90ml peptone and then transfer full 100ml into TSB(if it is said to transfer in TSB) or you can directly use 100ml of sample preparation, for further incubation.
Regards,
Aparna
I have to do 1:10 dilution. If I have to transfer all into TSB…then I have to dilute 100 mL into 900 mL TSB which is too many volume.
However can you explain these? ‘you can directly use 100ml of sample preparation, for further incubation.’
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RVSEB BROTH & XYLOSE LYSINE DEOXYCHOLATE AGAR USE FOR THE Identification for salmonella
hi
your not wrong in sample preparation(10g sample + 90ml peptone) but your wrong in transfer of 10ml,it should be full 100ml transfer.
Aparna
Ofcourse, here u have to pour or dilute 100ml … into TSB.