Speed (rpm) for incubator shaker is used

Microbiology
1

What speed (rpm) for incubator shaker is used to incubate test samples?

2

Hi,
Mixing depends on mixing speed and duration, varies for each make and model of shaker. You will need to establish and validate optimal parameters for your process.

3

Hi Claudia,

Thanks for your inputs. We have been trying to validate an incubator shaker that is going to be used for pre-incubating test solutions that are extracted from a medical device finished products for bacterial endotoxin testing.

Based on the current regulatory guidance which recommends that test solution should be pre-incubated at 37 C for not lest than 1 hour with agitation but there is no specific on what the speed (rpm) should be used. However, we conducted 3 trials with 75 rpm, 100 rpm, and 125 rpm and the results for all 3 trials are consistent and conforms with CV%<20. After 3 study trials, it shows no significant impact on the results for all 3 trials.

Thank you again for your inputs.

Kly