Rappaport Vassilieds Salmonella Enrichment Broth


It is because of the fact that higher temperatures other than 115 degrees can interfere with the selectivity of the medium. It is a selective medium for Salmonella species except for Salmonella typhimurium. The selectivity is due to malachite green, high pH and osmotic pressure.

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Because they contain double sugar molecules so you should not be autoclaved more than 115 degree celcius it will be denatured. That’s why the reason