Product BET testing by 50-50method

In product testing why we are using 4lamda? Why not we are not using 2lamda are lamda

Lamda is nothing but lysate sensitivity, 4lamda is u r increasing the sensitivity of ur lysate four times making ur lysate more efficient. There is no rule that u have to check as per 4lamda it depends on the endotoxin limit of your product and when u go for sensitivity confirmation of lysate then u have to do it for 4,2,lamda and more… That’s entirely a different story with different reasons…