Process Validation: should we manufacture the validation batches with different DS batches

In the scope a product transfer to another manufacturing site, we would need to validate the manufacturing process on site. Unfortunately, only 1 API batch is currently available and waiting on a second batch will delay the project. Would it be possible to manufacture all the three validation batches with only 1 drug substance batch?

Should be ok. Since you are not qualifying the raw material. it should be already qualified. the remaining is only manufacturing at diff site.