I have got some sources about the title but it seems there is lots of methods about it and im confused (I dont know what to do in my case)
I have ATCC microorganisms like P. aeruginosa, S. aerus, E.coli, Salmonella enterica…, C. sporogenez, B.subtilis, C. albicans which are all 4. passage kwiksticks.
As you know i can use 5. passage at last.
1- When i inoculate the kwicksticks on agar medium, the microorganisms on the agar surface are 4. or 5. passage?
2- What is the best way to preservation of these microorganisms. I have a procedure about microbank and it says i can preserve the microorganisms in microbank tubes until the end of the expiration date?
So, i have some old procedures and some says; inoculate the agar surface and keep the agar media in 2-8 C in refregirator, prepare subcultures and keep the subcultures in the same conditions, repassage the main inoculated media monthly but i dont think is it usefull or practical with the 4. passage kwicksticks.
So what i need?
2.-3. passage ATCC microorganisms?
Usage of microbank tubes?
Thanks for your advices…