On 90mm petri plates, How much minimum colony can be counted for fungi in term of CFU/mL ?

Depent on media, if you used SDA counted maximum 40 at 3-4 day

And on SCDA counted upto 60

Maximum 40 but what about minimum???

minimum could be absent for product if u inoculate organism follow factor of 2

Dear Sir,

I have a product containing culture of fungi.

Product says there is a 1 x 10^9 cfu/GM fungi content.

I am serially diluted the samples and plates on different fungi suitable medium.

Like potato dextrose agar,Sabroud agar etc.

So how can I achieve 1 x 10^9 cfu/GM content.

In 10^-8 dilution I got how much minimum colony count than I considered as 1 x 10^9 cfu/GM?

For example after serial dilution You are getting colonies in 10^8=10

Then u can take that as 1 x 10^9

**You can count colonies in dilutions which gives result less than 100 cfu.**

Example you are getting colonies in dilution 10^7=70cfu

Then u can take that as 0.7 x 10^9

In microbiology exact result is not possible.

Thank you so much for your response.

Is there any reference for counting fungi colony in less than 100 cfu ???

If there than plz suggest me.