Microbial Limit Test (PFS)

Which agents or method I applying to neutralizing penicillin antibiotics to highest recovery from products for enumeration test?

Aseptically dilute your sample with diluent containing beta-lactamase or penicillinase prior to performing your test

The neutralizer add before pouring the medium?

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If your goal is enumeration I would suggest membrane filtration technique instead of pour plate. If no other possibility then add neutralizer to sample or add it to the medium, before pouring


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