Microbial Limit Test (PFS)

Which agents or method I applying to neutralizing penicillin antibiotics to highest recovery from products for enumeration test?

Aseptically dilute your sample with diluent containing beta-lactamase or penicillinase prior to performing your test

The neutralizer add before pouring the medium?

1 Like

If your goal is enumeration I would suggest membrane filtration technique instead of pour plate. If no other possibility then add neutralizer to sample or add it to the medium, before pouring

By membrane filtration with 5 wash of neutralizer

Click Here