Microbial analysis of water in a pharmaceutical company

Hi,
I am working as a Trainee QC Microbiologist at a pharmaceutical company. As a part of normal routine I have to analyse the water for total viable count and total coliform. According to standard, the media used were Soyabean Casein Digest agar and MacConkey agar Pour plate method was followed and incubated @ 32°C for 24 hrs and 72 hrs.
My doubt is that why was the plates incubated for 72hrs?
Can anyone explain?

It doesn’t means 72 hours exactly , it means it range between 24-72 hours. Within that time most of bacteria growth occurs. While for fungus it take between 3-5 days.

1 Like

Dear Emmanuel,
Ok .Thank you for the clearing my doubt.
Regards,
Sudeep

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