Media fill filtration

At which temperature of wfi we should add scdm to manufacture 3% media fill.
At which temperature media fill filtration should be done.
What will be effect of overheat for media fill solution.

Add scdm at 55-60 degree. Centigrade and cool upto 25 degree and filter it.overheated may produce unclear dark brown solution and it may affect the growth property of media .

Yes, overheated media may lose its nutritional value.