If we change the method of standard preparation, do we need revalidation of the method. It is For terpenes validation by GC

Validation
1
  1. I have two different terpene standards. Prepared separately And did the validation by GC.
  2. Now I want to mix the two standards in single volumetric flask to save the time. So do we need to revalidate the method?
2

of course. You must revalidation all the request follow ICH such as specificity, linearity, precision, accuracy…