If we change the method of standard preparation, do we need revalidation of the method. It is For terpenes validation by GC

  1. I have two different terpene standards. Prepared separately And did the validation by GC.
  2. Now I want to mix the two standards in single volumetric flask to save the time. So do we need to revalidate the method?

of course. You must revalidation all the request follow ICH such as specificity, linearity, precision, accuracy…