HPLC Calibration

Quality Assurance
1

While HPLC Gradient Calibration- For LC2010 series- in A pump-100%water and in B pump- 3mL Acetone in 1L water used.
For LC2030 series 10 mg caffeine in 1L water used. My question is why different combinations are using? Can anyone explain…thanks in advance.

2

Actually Acetone Are in All Labs Bcoz Acetone Is Highly Absorbance and water Not have absorbance

3

Following are the reasons for utilising Acetone in water as solvent for HPLC Gradient calibration,

  • Acetone has UV- cut off at 330 nm
  • It is easily available in the purest form
  • It is less expensive
    Actually we can use use any other solvent in place of Acetone which has UV cut off above 250 nm.