How to test tlc for herbs?

How to prepare sample & mobile phase, i want to know details, pls help me.

You may consider HPTLC techniques (HighPerformance Thin Layer Chromatography) for separation of herbal compounds. Please refer Ayurvedic medicines pharmacopoeia or equivalent standard books for better understanding of mobile phase combinations, pre-treatment to herbal samples and techniques of chromatographic separation.

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How to calculate terotical plates in Gc

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