Gas chromatography

During analysis of Gas chromatography the area variation observed for sample.

Injection -01 observed 26 area
Injection -02 observed 375 area
Injection -03 observed 700 area

What is the root cause?

Dear Kiran,

Kindly check the calibration of your instrument is your injector linearity, precision passing the variation in the area has to be attributed to the injection malfunction as it is not able to deliver the exact volume.
If this is head-space issue then also check the valve that is controlling the split ratio.
But first check the calibration status of the instrument.