What is the difference between auto titration assay and hplc assay
Autotitration assays are the volumetric analysis based on the principle of potentiometric methods. While HPLC methods are chromatographic methods based on the principle of chromatography having staionaery phase and mobile phase. The analyte(s) to be determined are dissolved in the mobile phase and passed over chromatographic columns impregnated with the staionary phase at standardised conditions of flow rate and pressure. Depending upon polarity or Non-polarity characteristics of the analyte compounds and the stationary phase (like, C18 / C8 Silica), the analytes are quantitatively separated from the mixture (Test solution) and hold on the stationary phase and then get eluted quantitatively again in the mobile phase.