Deconatamination of used biohezard media in microbiology department, Safe disposal.
1-Use purified water to prepare solution of one of the following:
2- 5% Sodium hypochlorite
3- 3% Phenol
4- 70% Ethyl alcohol or Isopropyl alcohol.
5- Spray to disinfect the microbial waste by using one of the above solutions in (5.1.2).
6- Tight the plastic bag well, affix the "BIOLOGICAL HAZARD" label and then
7- place it inside a disposable yellow plastic drum covered by lid and labeled by "BIOLOGICAL HAZARD"
8- Transfer the drum to a dedicated biohazard room in scrap yard to be taken by the contractor for incineration.
9- Record the activity as per annexure
10 - Contractor must provide an incineration certificate which proofs that incineration has been
done properly.
11- An alternative method, decontaminate using autoclaving process.
Autoclaving:
12 - Carry out decontamination by autoclaving process
13- Dilute the liquid waste to about 1-10 with tap water and discard in the sink drain with
running tap water.14-
14- Collect the decontaminated solid waste in a sterilizable bag and disinfect by using one of the following: 5% Sodium hypochlorite, 3% Phenol and 70% Ethyl alcohol or Isopropyl alcohol.
15 - Make Cycle on the Empty chamber to confirm the Decontamination process and decrease the
16- a load of microorganisms if it is high.
17-. Decontamination by 5% Sodium hypochlorite, 3% Phenol and Ethyl alcohol or Isopropyl alcohol 70 % .
18- Tight the plastic bag well, affix the “BIOLOGICAL HAZARD” label and transfer to scrap yard at the end of shift.