Deconatamination of used biohezard media in microbiology department, Safe disposal.
1-Use purified water to prepare solution of one of the following: 2- 5% Sodium hypochlorite 3- 3% Phenol 4- 70% Ethyl alcohol or Isopropyl alcohol. 5- Spray to disinfect the microbial waste by using one of the above solutions in (5.1.2). 6- Tight the plastic bag well, affix the "BIOLOGICAL HAZARD" label and then 7- place it inside a disposable yellow plastic drum covered by lid and labeled by "BIOLOGICAL HAZARD" 8- Transfer the drum to a dedicated biohazard room in scrap yard to be taken by the contractor for incineration. 9- Record the activity as per annexure 10 - Contractor must provide an incineration certificate which proofs that incineration has been done properly. 11- An alternative method, decontaminate using autoclaving process. Autoclaving: 12 - Carry out decontamination by autoclaving process 13- Dilute the liquid waste to about 1-10 with tap water and discard in the sink drain with running tap water.14- 14- Collect the decontaminated solid waste in a sterilizable bag and disinfect by using one of the following: 5% Sodium hypochlorite, 3% Phenol and 70% Ethyl alcohol or Isopropyl alcohol. 15 - Make Cycle on the Empty chamber to confirm the Decontamination process and decrease the
16- a load of microorganisms if it is high.
17-. Decontamination by 5% Sodium hypochlorite, 3% Phenol and Ethyl alcohol or Isopropyl alcohol 70 % .
18- Tight the plastic bag well, affix the “BIOLOGICAL HAZARD” label and transfer to scrap yard at the end of shift.