Culture Maintenance

My question is why we use SCDM 10% Glycerol for culture work

Scdm with 10 %glycerol… totally new combination… I have never heard about this…

1.0% glycerol protracts the media from cracking.

Not 10% we use SCDA plates with 1% or 0.5 % glycerol for settle plate exposure

Yes. 1percent glycerol to avoid dehydration. Mainly for settle plate. and not that to exposed plate. It should be added while preparing media before sterilization.

for storage of culture at defrezzer for max. time.