Please tell me anyone bracketing standard concept
To ensure the total samples analysis completed without any interruption.
i.e; During the analysis there are errors occurred like pressure fluctuations, column leakage etc…
Bracketing standard has to compared with system suitability injections to ensure the entire analysis completed without any interruptions like above mentioned.
Because to know the hplc / GC system is working perfectly till the last sample.
Actually, System Suitability is run to show that the system is working perfectly. The purpose of “Bracketing Standards” or “Check Standards”:
There are times when the HPLC / GC conditions can change during a sequence of samples. The longer the runtime and the more samples in the sequence, the greater the likelihood of this happening.
Sometimes these changes affect the detector response, and hence affect the validity of the calibration. We can monitor changes by running a QC standard periodically through the sequence, but this does not update the calibration. We can re-run the calibration standards periodically during the run, but this will either average with the previous calibration or replace it, and either way, it means that every few samples, the calibration changes, making it hard to compare results. We could ignore the changes, but this means that the calibration accuracy becomes progressively worse during the sequence. The solution is to use bracketed calibration.
Essentially this means running the calibration standards at the beginning of the sequence and at the end, and makes the assumption that any changes occurred in a linear manner during the sequence. The data system then changes the calibration incrementally from the beginning to the end, and applies this to the results. If the assumption that the change was linear is correct, the data should then all be correctly quantified. Not all data systems have this function, and for long runs it is very useful.
You can also prepared SOP for HPLC / GC analysis with incorporate bracketing standard when required.
For Example : As per below table