Hello all of u. Plz clear my doubt. How to calculate MVD of any raw material bet test.
NMT 5.0 ENDOTOXIN unit PER GRAM of sodium chloride. How much gram sodium chloride diluted and how to find mvd
If you are provided with 100mg or 0.1gm of sample and your endotoxin limit is NMT 5.0EU/gm
MVD = (5.0EU/gm ÷ lysate sensitivity ) × 0.1gm
For example: if ur lysate sensitivity is 0.03EU/mL
The MVD= (5.0EU/gm ÷ 0.03EU/mL) × 0.1gm
= 16.6667
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O.1 gm nacl dissolve how much LAL water for dilution
Some time my clot is break whats region for it
trial and error… but I think 1 mL should suffice, also easiest in calculations
different question than the MVD, impossible to answer without more info. sample type, sample prep etc
Do you have any reference regarding this?
Ph.Eur. 2.6.14
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1g of sample dissolved in 10ml of LRW to obtain the concentration of 100mg/ml
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