Hey guys, im working in a microbiology lab. and we are going to start analysis of purified water ;
I have multiple questions ;
After the filtration of sample, is it necessary to wash the membrane with peptone water?
Which method is the better for detection of P. aeruginosa in purified water?
a- Filter 100ml of purified water, add it to the TSB media incubate for 1 day, take passage to Cetrimide agar
b- Add 10ml of purified water directly to 100ml of TSB media , incubate for 1 day, take passage to Cetrimide agar
Thanks for your helps…