Sterilizations SIP for bioreactors

What does “better sterilization” mean to you?

In terms of sterility assurance, and assuming this is moist heat sterilization, the process lethality (F0) can be used as a measurment of process effectiveness. More lethality gives better sterility assurance (SAL).

The first option: 124°C for 15 min, has more lethality (F0 = 29.2 min), than the second option (F0 = 5.9 min); so for sterility assurance, the first option can be considered “better sterilization” in terms of process lethality.

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