Why blank soln injected in hplc
For confirmation of diluent peaks in sample.
Thanks mam for giving replying
Might be Column conditioning and stable base line
To check interference of diluent at the RT of interested peaks.
With the help of blank solution we can distinguish peak from blank and sample
Peak is eluted in blank solution and same RT Should be eluted in sample solution you integrated all peak name as blank. Except main peak
Blank solutions are injected into a HPLC system to stabilise the system and to remove any peaks that may interfere with the API or Drug Product you are injecting , made in the same BLANK solution.