Pharmaceutical water validation

Can anyone suggest protocol or guideline for pharmaceutical water validation( microbial testing)?

  1. When we determine Reynolds number for pharmaceutical water during water system validation or after validation?

Microbiological testing is done according to relevant water monograph (PW/WFI) in Pharmacopeia
Reynolds number is usually determined as part of system qualification.

Thanks, but within microbial testing what is perform during validation. can I get some more details or references.

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