Necessity of SCDM sample suspension incubation for 2 hrs at 20-25 before inoculation into EEB media

Is it necessary to incubate the SCDM sample suspension at 20 - 25 degree centigrade before inoculating in to EEB media?

Yes this is must because this is regulatory requirement.

Thank you raj… can u please tell me in which regulatory it was mentioned.

United states pharmacopea(USP)

chapter number pls…

61 and 62

This is found in USP 61 and 62 and IP 1 volume chapter 2.2 biological methods pls go to refer