HVAC validation protocol

What to perform in HVAC Validation?
Than Answer is your User Requirement Specification, which includes requirement.
For the requirement you can refer WHO guideline : http://www.who.int/medicines/areas/quality_safety/quality_assurance/HVAC_Part2_QAS18-759-15022018.pdf?ua=1
above is under draft, but still you can find the requirement.
Test Methodology, Report and criteria , you can refer ISO 14644

Ideally Protocol should cover below requirement (But not limited to) :

  1. Objective
  2. Scope
  3. Responsibility
  4. Training Participation
  5. Instrument detail to be used for the Validation activity and their status
  6. SOP detail
  7. Acceptance criteria of the test
  8. Methodology/Procedure of the Test
  9. Reporting Result
  10. Non Compliance observed (if any)
  11. Summary and Conclusion
  12. Approval of the protocol

Some industries follow Pre Approval and Post Approval concept. but it may vary