We know that, during Media Fill of aseptic processing, filled container/vials/ampules are incubated from low temperature to high temperature. But recently I have saw a article (Incubation conditions for Fungus and Bacteria using Common Media) of your site where you mentioned with cause that the incubation should be started from high temperature to low temperature.
So, it seems to me contradictory and I will be grateful if you answer me as soon as possible about the reason of incubation during Media fill validation.
Md. Rasel Bhuiyan