Disinfectant efficacy testing

during disinfectant efficacy study in pharmaguideline there is no use of any neutrilizer. so my question is if we are not using any neutrilizer to neutrilize the activity of disinfectant how are we sure that we can have the result?

@vivek neutralizer is not required in disinfectant efficacy test because we also take sample from floor without apply disinfectant.

Dear sir
Please explain